SERVICES
Vector Design
The first step in our protein production process leverages our expertise in vector design to produce functional proteins.
- Codon Optimization: We optimize the gene's codon usage to match the host expression system, enhancing translation efficiency and protein yield.
- Gene Synthesis: After codon optimization, we synthesize the gene to meet optimal protein expression requirements.
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Subcloning: Subclone genes with
specific characteristics to tailor the expression vector to your
needs, such as:
- Signal Peptides: Facilitate protein secretion for easy recovery and purification.
- Cleavage Sites: Include cleavage sites to allow for the removal of affinity tags after purification, ensuring the final protein product is tag-free.
- Affinity Tags: Simplify protein purification and detection with tags such as HisTAG, FlagTAG, and StrepTAG (or no TAG!)
- Protein Marker Tags: Include tags to mark the protein, such as c-MYC and AviTAG.
Production
Our expertise spans the expression of various protein types—including enzymes, antibodies, membrane proteins, cytokines, and other challenging proteins—across different cell systems like bacteria, yeast, mammalian cells, and acellular systems, ensuring successful production for projects involving therapeutics, diagnostic tools, or research materials:
- Vector Options: Utilize either a vector you supply or one that we design and produce, ensuring optimal expression in the chosen system for reliable results.
- Scalable Production: From small to large scale, our platform adapts to your project needs, providing flexibility and efficiency at every production stage.
- Parallel Testing: Our multi-system platform enables simultaneous testing across different systems, allowing us to identify the best expression conditions for your protein quickly.
- Optimization: Extend production conditions within a single system to refine and determine the best yield and functionality, ensuring the highest quality output.
Purification and QC
Our expert team ensures the integrity and high quality of your proteins, whether soluble or membrane-bound, by developing efficient processes with optimized steps to meet your specifications.
- Customized Purification Strategies: Depending on your needs, proteins can be purified by affinity using tags added during the molecular biology phase (e.g., HisTAG, GST, or StrepTAG).
- Tag-Free Purification: For applications requiring tag-free proteins, we employ various purification methods, including:
- Ion Exchange Chromatography: Separates proteins based on charge differences.
- Size Exclusion Chromatography: Isolates proteins by size.
- Hydrophobic Interaction Chromatography: Utilizes hydrophobicity to separate proteins, ideal for proteins with exposed hydrophobic regions.
In terms of QC, we offer SDS-PAGE, UV and BCA quantification, Western Blot, as well as SEC, SEC-MALS, Circular Dichroism, Nano DSF, DLS,MS...
E.coli
E. coli is a popular choice for recombinant protein production due to its simplicity and cost-effectiveness.
- Fast Growth: Experience rapid production cycles with our E. coli system, minimizing your wait time.
- High Yield: Maximize your output with high-yield production capabilities.
- Scalable: Easily scalable and adapted to your needs.
- Cost-Effective: Save on costs with low media and cultivation expenses.
- Robust: Our yeast systems tolerate a wide range of conditions, offering flexibility in your production process.
Adapted for a wide range of proteins: such as insulin, growth factors, interleukines, antibodies fragments, enzymes like Taq polymerase...
Mammalian Cells (CHO and HEK)
Mammalian cells are ideal for producing proteins with complex post-translational modifications.
- Post-Translational Modifications: Achieve accurate glycosylation and folding for functional proteins.
- Human-Like Proteins: Produce proteins that closely mimic those in the human body, ensuring biological relevance.
- High Quality: Ensure your proteins are functional and biologically active.
- Reliable: Trust in a widely-used and well-characterized system for consistent results.
Adapted for proteins like: monoclonal antibodies, enzymes, erythropoietin (EPO), and therapeutic proteins like interferons.
Yeast (S. cerevisiae and P. pastoris)
Yeast systems offer a balance between the simplicity of E. coli and the complexity of mammalian cells.
- Eukaryotic System: Benefit from eukaryotic post-translational modifications for complex proteins.
- High Yield: Achieve high protein yields with our optimized yeast expression protocols.
- Rapid Growth: Faster growth than mammalian cells, without sacrificing quality.
- Cost-Effective: Lower cultivation expenses than mammalian systems.
- Robust: Our yeast systems tolerate a wide range of conditions, offering flexibility in your production process.
Adapted for a wide range of proteins - antibody fragments, enzymes, antigens like hepatitis B surface antigen, and any other protein requiring eukaryotic post-translational modifications...
Acellular System (Wheat Germ Extract)
Acellular systems are advantageous for producing complex proteins.
- Cell-Free System: Simplify your process with no need for cell cultivation, no more toxicity during cultivation! This open system allows addition of additives, thus creating an environment favorable to protein folding.
- Rapid Production: Get your proteins quickly with fast synthesis times (1 week for large-scale production)!
- High-throughput productions available!
- Efficiency: Directly use DNA templates for efficient protein production. A powerful technology to make proteins that were previously difficult to produce.
Adapted for proteins like: Membrane
proteins, cytotoxic proteins, complex folding proteins, viral
proteins antigen for antibody generation and more broadly proteins
which are difficult to express in classic systems.
Let's merge our expertise !
Discuss your project with our specialists and find the best solution for your protein needs.